It was tuned on the plate out of the instrument, sounded different there also. The bandoneon I tuned last year was also tuned out of the instrument, then fine tuned within: in both instances the instrument had the harmonica sound, before and after tuning, the sound I associate with tango music, very different to when tango is played on accordion. Sounded like this on youtube: Astor Piazzolla - Bandoneón
Well then, it is *known* that several factors influence differences in *tone* from different reeds - different tongue material and shape, different shoe material and shape e t c . Maybe systematic research has been done, but reports seem to be few. So David, go ahead! Some kind of tone spectrometric registration and documentation is necessary to make findings objective. Sadly enough our ears sometimes are more sensitive than the equipment or at least interpretaton of technical findings vs audible sensations is a challenge.